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Cattle
milk progesterone tests
Heat detection is the key to profitability
Accurate timing of insemination
· Reduces calving to conception interval
· Reduces culling due to failure to conceive
Farm surveys found up to 20% of services
were wrongly timed, and with 20-25% of heats
silent, detection can be a challenge. |
On farm use of progesterone resulted in:
· An 18% increase in the number of cows
served after calving
· An average reduction of 7 days in the
calving to first service interval
· A 9% reduction in culling due to failure
to conceive
· A saving of approximately £85 per cow¹
Our milk progesterone tests demonstrate
where a cow is in the oestrus cycle and
deliver up to 98% heat detection. Using this
as a tool can help you to achieve your own
targets.
¹Work conducted in 1990 and saving
updated using inflation calculator |
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Ridgeway "M" Kit |
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Ridgeway "Dropper" Kit |
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Target
Kits |
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Ridgeway Milk Progesterone
Enzymeimmunoassay Procedure |
1.
2.
3.
4.
5. |
Bring to room temperature and strip
foil from required number of wells.
Empty wells and tap dry onto tissue.
Add 10µl of standard or sample
to each well.
Add 200µl progesterone-enzyme
label.
Leave 1-1.5 hours at room
temperature.
Wash 3 times with cold water
- tap dry on paper each time.
*
Add 200µl substrate in
substrate buffer. To avoid
contamination use a clean tip
Leave 10 min - 1/2 hour.
Compare colour development of
samples with standards by eye.
Strong colour - low progesterone ?
heat, not pregnant
Weak colour - high progesterone ?
mid cycle or pregnant
(Or read at 570nm on plate reader:
alternative substrate Sigma 104 at
405nm: 140mg/25ml buffer).
Samples
Whole bovine milk. May be stored at
4°C after addition of Lactab MkIII
tablets.
Standards
Progesterone in milk from an oestrus
or ovariectomized animal.
WARNING
All solutions contain thimerosal
(0.01% w/v).This is toxic. Dispose
of carefully and wash down sinks
with excess water. Keep away from
food and children and wash your
hands after use.
*Dissolve
substrate tablets in 25ml substrate
buffer 2 and mix.
Takes 20-30 minutes to dissolve.
Sufficient for one plate. Store in
the dark.
Do not contaminate with
progesterone-enzyme label 1. |
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Address: Ridgeway
Science, Park Farm Buildings, Park
Lane, St Briavels, Glos, GL15 6QX |
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