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Progesterone Assays

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RIDGEWAY - Advice on using On-Farm Progesterone assays
 








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Progesterone is produced by the ovary and is high at mid-cycle and in pregnancy. It is low at heat or if the cow is anoestrus. Milk progesterone test kits are a simple and economic method of measuring progesterone in milk. Strong colour is produced with low progesterone and a slight colour with high.
 
Progesterone assay may be used in a number of ways to investigate or improve reduced fertility in a dairy herd.
 
Sampling at day of observed heat. All samples taken on this day should show low progesterone. Samples from 20 cows would amply determine if heat were being wrongly interpreted.
 
Sampling at day 19 after service. If it has previously been determined that heat is being correctly identified (see above) sampling on this day will give early warning of return to service in 8/10 cows by showing a low level of progesterone: such cows should be carefully observed for return to service.
 
Sampling at day 24 after service. Cows apparently pregnant at day 19 (above) should be re-tested at day 24: this is statistically the best day for determining positive pregnancy but used on its own will only identify most non-pregnant cows too late for service at that heat.
 
Weekly sampling. Samples taken on a weekly basis can give information on whether cows are showing regular heat cycles: cows should show a low level of progesterone indicative of heat at least once in 3-4 samples. On the other hand if all samples are low the vet should be consulted about anoestrus. This will occur in some cows after calving. If all samples are high the vet may conclude that there is a cystic corpus luteum (but beware, the animal may be pregnant!). If evidence of normal heat cycles is seen, then heat detection may be slipping.
 
Heat Prediction. Samples are taken every other day from day 15 or 16 after a previous heat (or low level of progesterone during weekly sampling). When a new low level of progesterone is seen cows are observed closely for signs of heat or a "Kamar" device is affixed. Alternatively, the cow is served "blind" on the day after the low value. This may result in increased double services if the cow is seen bulling on a following day.

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